Preparation and Assessment of Antimicrobial Property of Resin Based Composites Impregnated with Proanthocyanidin and Titanium Dioxide

Mumtaz ul Islam                  BDS, M.Phil
Sardar Muhammad             MBBS, DOMS, M.Phil
Fazal Ghani                         BSc, BDS, MSc, CMP, PhD, FDSRCPS (Post Humous)


OBJECTIVE: To synthesize three experimental resin based composites (RBCs) out of a commercial preperation (Control)
by impregnating into each a fixed weight (0.01%) of titanium dioxide (TiO2) and three different weight % (0.01, 0.02 and 0.03)
of proanthocyanidin (PA) and to compare antibacterial property, from the zones of inhibition of streptococcus mutans
(S. mutans), of the specimen restorations made in the experimental and control RBCs.
METHODOLOGY: In the first phase, Streptococcus mutans were isolated and identified from the oral cavity of patients and
identified on the basis of morphogenic appearance of colonies and confirmed through catalase test and microscopic examination.
Isolates were then incubated and cultured for sensitivity. Specimens for control and experimental RBCs were made through a
metallic mold having 2mm thickness and 5mm diameter for all the 4 groups. Specimen restorations made in the commercial
as supplied RBC acted as the control (Group A). Specimen restorations in the Group B, C, and D were made in each of the
experimental RBCs modified with 0.01% proanthocyanidin (PA) and 0.01% titanium dioxide (TiO2), 0.02% PA and 0.01% TiO2
and 0.03% PA and 0.01% TiO2. The antimicrobial property of all the specimen restorations in all the groups was evaluated by
Bauer-Kirby (Disc Diffusion) method through the formation of zones of inhibition of S. mutans colonies on blood agar plates.
Data were computed using SPSS version 21 for descriptive statistics and analyzed using one-way ANOVA with p value 0.05
taken as significant.
RESULTS: The range of the zone of inhibition for specimen RBC restorations (N= 12) containing PA and TiO2 was 0.00 for
Group A, 10-12mm for Group B, 12-14mm for Group C and 9-10mm for Group D. The mean inhibition zone for the experimental
restoration specimens was 11±2mm for Group B, 12.7±1mm for Group C and 9.7±1mm for Group D compared to no inhibition
zones (0.00 mm) with the control RBC Group A. The order of the inhibition zones from maximum to minimum was
Group C > Group B > Group D > Group A. The differences between the mean values for the control and the experimental RBC
restoration specimens were significant (p < 0.000). The specimen restorations in the Group D appeared under cured and could
impact on their mechanical properties.
CONCLUSION: Irrespective of the mentioned concentrations, impregnating PA and TiO2 rendered the RBC antimicrobial.
RBC restorations made with the experimental RBC with 0.02% PA and 0.01% TiO2 proved relatively more effective in terms
of antimicrobial effect.
KEY WORDS: dental materials, resin based dental restorations, antibacterial resin based composite, proanthocyanidin, titanium
HOW TO CITE: Islam MU, Muhammad S, Ghani F. Preparation and assessment of antimicrobial property of resin based
composites impregnated with proanthocyanidin and titanium dioxide. J Pak Dent Assoc 2021;30(4):243-248.
Received: 27 January 2021, Accepted: 17 August 2021

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