Comparison of Remineralization Effect of Casein Phosphopeptide Amorphous Calcium Phosphate and Sodium Fluoride on Enamel Surface after Orthodontic Debonding: An In vitro study

Hafiza Asma Jawaid1           BDS, MDS
Asmi Shaheen2                    BDS, M.Phil, FCPS
Muhammad Ilyas3                BDS, FCPS
Waheed-ul-Hamid4               BDS, M.Orth RCSED, MS (Ortho)
Ahmad Shamim5                  BDS, MDS
Asif Shakoor6                       BDS, M.Phil



OBJECTIVE: To compare the result of "casein phosphopeptide amorphous calcium phosphate" with "sodium fluoride"
(900 ppm) in remineralization of enamel after orthodontic debonding.
METHODOLOY: This study was performed on premolars. Teeth were distributed into 3 groups A, B and C. The baseline
microhardness of samples was measured. To create artificial demineralization, all samples were dipped in demineralizing
solution. Surface microhardness (SMH) was re-measured for demineralization. Then, CPP-ACP was applied on teeth of gp B
and NaF (900 ppm) was applied on teeth of gp C for remineralization. All teeth were cycled between deionized water and
surface treatment for 12 weeks. The SMH of gp B and gp C was re-measured for remineralization. Paired t-test and Independent
t-test were practiced to match MH before and after demineralization while keeping P value < 0.05. One way ANOVA was
practiced to match mean difference in baseline MH and after demineralization.
RESULTS: The baseline SMH of enamel decrease after demineralization. After application of test materials, mean SMH
increased by 230.2 VHN and 207.5 VHN respectively. The %age of SMHR reveal the recovery rate of 41.2% in CPP-ACP and
19.1% in NaF group.
CONCLUSION: For treating post orthodontic demineralized lesions, a remineralizing cream having casein phosphopeptide
stabilized amorphous calcium phosphate is useful with some mineral and aesthetic improvements.
KEYWORDS: Casein Phosphopeptide Amorphous Calcium Phosphate (CPP-ACP), Sodium Fluoride (NaF), Surface
Microhardness (SMH), Vickers Hardness Number (VHN), Rmineralization.
HOW TO CITE: Jawaid HA, Shaheen A, Ilyas M, Hamid WU, Shamim A, Shakoor A. Comparison of remineralization effect
of casein phosphopeptide amorphous calcium phosphate and sodium fluoride on enamel surface after orthodontic debonding:
An in vitro study. J Pak Dent Assoc 2022;31(2):65-69.
Received: 06 October 2021, Accepted: 27 March 2022

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